Notes from the coordinating meeting of collaborators


Date: Tuesday, October 4, 2011
Place: CTU - Faculty of Biomedical Engineering (+ 301,305,307)
Participants: David Birch, Vladimír Blažek, Petr Brůža, Vlastimil Fidler, Martin Hof, Alexandr Jančárek, Taťána Jarošíková, Patrice Jichlinski, Pavel Kučera, Norbert Lange, Radek Macháň, Klaus Mann, Vlastimil Matějec, Dalibor Pánek, Marie Pospíšilová, Christoph Rose-Petruck, Patrik Španěl, Pavel Vrba, Miroslava Vrbová, Sergey V. Zakharov, Vasil S. Zakharov

Morning session (in Room No. 615)

3 working group established:

  • G1 (related to Key activity KA2 – reporter M. Vrbová)
  • G2 (related to KA4+5+7 – reporter V. Fidler)
  • G3 (related to KA6+8 – reporter P. Kučera)

Discussion in working groups (Rooms No. 301, 305, 307)

  • Possible cooperation among KA
  • Up-dating of working plans
  • Students projects suggestions
  • New topics to be involved

Afternoon session (in Room No. 615)

Reported conclusions of the working groups:

G1 (M. Vrbová)

Research activities

  • Established long term cooperation among partners from EPPRA (France), LLG (Germany), IPP AS CR and CTU (FNSPE, FBME) will continue.
  • Common goal is to increase the brightness of both (capillary and laser plasma) XUV radiation sources which are developed.
  • Special attention will be paid to nitrogen plasma sources generating monochromatic radiation at 2.88 nm.
  • Common publication, integrating the results presented at this Workshop 1 will be prepared.
  • XUV emission from picoseconds laser plasma source will be modelled.
  • Solid target (ceramics BN) will be judged as an alternative.
  • Cooperation with the group G2 is agreed on the development of imaging methods (C. Rose-Petruck), pump –probe fluorescence experiments (V. Fidler), and NEXAFS method development (D. Pánek).

Education

  • Topical XUV seminar will be organized next autumn (2012).
  • Š. Vondrová, new student in PhD program at FBME will contribute to laser plasma system optimization.
  • J. Novák, diploma student of FNSPE will perform pilot roentgenography experiments with capillary discharge source.
  • Suggested themes for master program students:
  • XUV radiation source and its application in biomedicine (A. Jančárek, FNSPE)
  • Space-time diagnostics of  XUV radiation pulses  (M. Vrbová, FBME)

G2 (V. Fidler)

  • The “cross-linked” research activities of  KA4 and KA5 will focus at the following three fields:
  • “Tool box” for imaging (including nano-particles and scattered imaging). (C. Rose-Petruck, D. Birch, V. Fidler)
  • Aggregation phenomena (including selected protein aggregation, model membranes and molecular monolayers). (D. Birch, V. Fidler, M. Zimmt)
  • Metabolic monitoring (new field with participation of  P. Španěl from Heyrovsky Institute)

=> corresponding topics for Mgr. & PhD students were submitted to M. Vrbova

Closer interactions with KA2 is expected in 2012/13 (P. Kučera), as well as with new activity KA9 (V. Blažek); in the meantime, depending on the capillary X-ray source, with KA2 (S. Jančárek).

Education
PMB education needs to strengthen in molecular sciences – we recommend to review this part of the study programme this year and to propose changes next year.

  • We recommend introduction of a new course “Mass spectrometry” into curricula (in English, given by P. Španěl).
  • General introductory course on optical (molecular) spectroscopies would be advantageous for all students oriented to the three area mentioned above – suggested for further attention.
  • Prof. D. Birch proposes preliminary table of the courses for the above three tracks.
  • Topical seminar focused at KA4 and KA5 will be organized in spring, 2013.
  • Topical condensed course on X-ray-matter interaction and imaging will be given by C. Rose-Petruck in January 2013 (M.Vrbová and C.Rose-Petruck).

G3 (P. Kučera)

Research will be aimed to improvement of the existing and developing of new approaches to phototherapy.

  • A close collaboration between KA6 and KA8 has been decided (foreseen for the 2nd/3d year).
  • In cooperation with FBMI CTU, IPE AVCR, v. v. i. and ICPF AVCR established OF laboratory on FBMI during 2nd/3d years of Project. (Mainly laboratory equipments for development OF fluorescence sensors (spectral analyzer, optical hardware, PC and suitable software. responsible person: MP, GK, VM, TJ) .
  • Cooperation KA8 with KA7 in spectroscopy of materials suitable for OF fluorescence sensors (responsible person: MP, RM)

Education
The following student research projects have been proposed:

  • Endoscopic monitoring of ciliary beating frequency of the upper airways epithelium
    Purpose: improving of endoscopic diagnostics in oto-rhino-laryngology, pneumology and pharmaceutical research.
    (Submitted to students as a diploma study, responsible: M. P. and P. K.)
  • Development of UV transmitting optical fibers
    Purpose: complement to cystoscopy – measurements of NADH fluorescence during phototherapy of bladder cancer.
    (Responsible: V.M., duration till July 2012)
  • Selective irradiation of tumoral cells during cystoscopy
    Purpose: major instrumental improvement of cystoscopic photodynamic therapy.
    (Responsible: V.M., M.P., N.L., P.J., P.K., duration about 2 years, will be proposed for PhD theses).
  • “BLA3DDER”
    Purpose: conception of a reference system allowing precise and reproducible positioning of cystoscopes and cartography of the urinary bladder cavity
    (Responsible: from FBMI to be defined - experience with position sensors, P.K., P.J., N. L.)
  • Variations of NADH fluorescence induced by PpIX photodynamic therapy
    Purpose: to answer the question whether the NADH is a suitable candidate for on-line monitoring the cell metabolic state during the PDT.
    (Responsible: P.K., M. Vrbová)
  • Four topic Projects for Mgr. students are suggested in field of OF sensors  (KA8) including cooperation with FBMI and IPE AVCR, v. v. i. and ICPF AVCR, v. v. i. (responsible persons: MP, VM, GK, TJ)

A seminar/course on “Photosensitizers” could be organized during 2012 springtime. (Responsible: N.L.).


Conclusions

  • Concluding discussion of all participants confirms that the Workshop 1 strengthen the collaboration among CTU project participants, theirs Czech partners (Institute of Plasma Physics AS CR,  J. Heyrovsky Institute of Physical Chemistry AS CR, Institute of Photonics and Electronics AS CR, Institute of Chemical Processes Fundamentals AS CR) and foreign colleagues from EPPRA s.a.s., France ,Laser Laboratorium Goettingen e.V., Germany Department of Chemistry, Brown University, USA , Stratclyde University, UK , Dept. of Urology, University Hospital, Lausanne, Switzerland  and Laboratory of Pharmaceutics and Biopharmaceutics, University of Geneva, Switzerland.
  • A new key activity (KA9), dealing with interaction (diffraction, absorption, reflection) of optical beams with tissue was suggested by prof. V. Blažek.
  • Research problems, which may be collaboratively solved, were formulated.
  • Tentative research themes for the Mgr. and PhD students were judged by all participants and will be worked out in details during coming semester.
  • Preparation of special lectures and courses for Mgr. and PhD. program were discussed and agreed.
  •  Forthcoming topical seminars are planned:
  • XUV sources and applications - autumn 2012
  • Biomedical imaging and  new combined TR/OPT/UV/XUV spectroscopy – spring 2013
  • Seminar on  OPT – Tissue interaction and “Photosensitizers” – autumn  2013
  • Workshop 2 – final meeting in the frame of this project is planned for spring 2014.


October 10, 2011  M. Vrbová, V. Fidler, P. Kučera